Bio I Lab Report 3

General Biology I, Spring 2012 Lab Report 3
puddle: Adam Porter
Date: 4/26/2012
Lab: colloidal changeatin Electrophoresis
Purpose of this lab: The purpose of this experiment is to make gelatin electrophoresis and to understand how to use the results. Such as in cases where deoxyribonucleic acid is used to either exclude or confirm paternity or wickedness suspects.
Introduction: gelatin Electrophoresis is a process used to separate molecules found on their size and charge. This is accomplished by placing the molecules in a well inside the gel and electrifying the gel field. The molecules, which atomic number 18 now charged by the electricity, move across the gel. The shorter molecules pass on move more easily through the pores of the gel and because of this they provide move further than the longer molecules. This is called sieving.
In this experiment a pre-prep ared agarose gel is used. The gel is typically made with between 0.7% to 2% agarose which is dissolved in an electrophoresis buffer. Six contrary samples are used. The samples represent two DNA markers from blood at the crime scene as well as two DNA markers from each suspect. The positive electrode is set at the bottom of the gel because the DNA fragments are negatively charged so they get out move through the gel towards the positively charged electrode.




Materials:
--Pre-prepared agarose gel inside a gel box
--6 20 ul DNA samples
--pipette
--6 yellow-bellied pipette tips
--carbon fiber electrodes
--electrodes and indicant source
Methods: First each of the sestet DNA samples are loaded into separate wells inside the gel using a pipette with a different tip each time to avoid contamination. Make real which to record which samples are in each well. Place a carbon fiber electrode in each end of the gel box so that they are touching the buffer solution. capture the electrodes to each end of the box and to an unplugged power source. Set the power source to 40 volts, plug in and turn on. live 20 to 30 minutes and record results.
Results:
Discussion:

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