Analysis of the Enzyme Sucrase for Optimum Temperature, pH, and Concent

Analysis of the Enzyme Sucrase for Optimum Temperature, pH, and Concentration schemaEnzymes react contrastively under different conditions and concentrations, being the most arable at the enzymes specific best condition and concentration. The enzyme sucrase, extracted from yeast, breaks down the complex dulcorate saccharose into the simple sugar glucose. Testing for sucrases optimum milieu, multiple reactions were ran using varying tot ups and concentrations of sucrose and sucrase at different pHs and temperatures. The product was accordingly treated with Benedicts solution to visu altogethery observe what amount of glucose was confront after the reaction was ran negative results being little to no glucose present and positivist results being glucose present. The varying levels of colors created by the Benedicts trial were recorded as 1 (negative) blue 2 green 3 yellow 4 (positive) orange and 5 (positive) red. After running the reactions to insure the optimum temperature and pH, 37 degrees Celsius was determined to be the optimum temperature while pH 2.0 was determined to be the optimum pH. This means that under these conditions the enzyme will garter produce the most product from the substrate. After finding the optimum conditions the dickens different concentrations of sucrose were ran for ten minuets taking an initial sample and then subsequent samples every minuet, under those conditions, one concentration at 2.5% sucrose and again at 10% sucrose. The 10% sucrose had the most positive results because there were sufficient active sites to bind with most of the sucrose present producing glucose more efficiently than the 2.5% sucrose. As with the 2.5% sucrose all of the sucrose was adhesion to the active sites of sucrase and there were ... ...ty Benedict test results. One of these possibilities or some combination can lead to inaccurate optimum temperature and or pH, which in turn would through off the optimum concentration. Over all I feel tha t the most important part of the optimum environment is the pH because of the competitive inhibition caused by free protons in solution. whole kit CITEDHalverson, K. M. and B. A., Barry. 2003. Sucrose and glycerol effects on photosystem 2. Biophysical daybook 85 1317Lawrence, S., M. K. Heidemann and D. O. Straney. 2006. biologic Sciences 111L Laboratory Manual. Hayden-McNeil Publishing, Inc., PlymouthPeluffo, D. R.. 2004. Effect of ADP on NA+-NA+ Exchange chemical reaction Kinetics of Na,K-ATPase. Biophysical Journal 87 883Shaevitz, J. W., S. M. Block, and M.J. Schnitzer. 2005. Statistical Kinetics of Macromolecular Dynamics. Biophysical Journal 89 2277