INTODUCTION:
Bacteria are prokaryotic cells; simple organisms that rest of one double stranded circular Deoxyribonucleic acid ( deoxyribonucleic acid) molecule. bounty in most bacteria cells are extra circles or sometimes linear piece of desoxyribonucleic acid called plasmids. Bacteria cells sick in an asexual fashion, undergoing binary fission, which simply mean they double up their own DNA. Thus, they lack genetic form in their alteration in their population because they clone themselves when they reproduce. This poses a big disfavor to them because without genetic variation of a population genetic variation a population evokenot respond to changing environmental variables. As a result, they face the risk of extinction, though bacteria poop gain new genetic information through the transit of genetic recombination. There are three mechanisms of genetic recombination; transduction, sexual union and transformation. In this lab we will be focusing on transformation of a bacterium, Escherichia Coli (E.coli), with a recombinant plasmid.
Transformation is the up bundle of DNA directly from the environment.
After a cell dies, it lyses and releases DNA which is called raw(a) DNA. If naked DNA is recognized by cell-surface receptors, the DNA can be taken up, but only at a certain stage of the cell cycle when the cell jetty is not completely synthesized. For transformation to actually occur there should be similarities between the donor and recipients DNA because cell-surface receptors are specific, therefore transformation is limited to closely related to species.
Since E.coli does not normally alter its cell wall to take up naked DNA, it has to be exposed to calcium chloride and temperature fluctuations. atomic number 20 chloride weakens the cell wall. And a process known as combust shock...If you want to get a full essay, order it on our website: Ordercustompaper.com
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